Chemotranscriptome analysis indicates the neurotrophic and neuromodulator effects of a citicoline molecule
https://doi.org/10.14412/2074-2711-2020-4-91-99
Abstract
Objective: to investigate the effect of citicoline (CTC) on gene transcription.
Material and methods. Chemotranscriptome analysis of the CTC molecule was carried out on an NPC.TAK model, provided that the cells were incubated with CTC for 24 hours.
Results and discussion. CTC dose-dependently affected the transcription of 8,838 out of 12,716 annotated human genes, mainly by increasing the transcription of the genes involved: 1) in the neurotransmitter metabolism of serotonin (n=36), dopamine (n=32), GABA (n=14), and acetylcholine (n=27); 2) in showing the effects of neurotrophic factors (n=152), including nerve growth factor (n=11); 3) in maintaining the cardiovascular system (vasodilation and cardiac electrical activity; a total of 76 genes). CTC reduced the transcription of the genes, whose protein activity supported inflammation (n=86) and cell division (n=656). CTC elevated the expression of 60 genes involved in triglyceride processing and decreased the expression of 51 genes whose proteins were involved in cholesterol metabolism. CTC increased the transcription of the genes involved in the body’s response to various drugs, including antiepileptic drugs (n=20), dopaminergic agents (n=19), antipsychotics (n=38), anxiolytics (n=21), sedatives (n=22), antidepressants (n=35), anesthetics (n=23), and antidementia drugs (n=11).
Conclusion. Chemotranscriptome analysis indicated the positive effect of CTC on neurotransmission, neuroprotection, lipid profile, and a higher neuronal susceptibility to other neuroactive drugs.
About the Authors
I. Yu. Torshin
Institute of Pharmacoinformatics, Federal Research Center «Informatics and Management», Russian Academy of Sciences; Center for Big Data Storage and Analysis, National Center for Digital Economy, M.V. Lomonosov Moscow State University
Russian Federation
44, Vavilov St., Build. 2, Moscow 119333
27, Lomonosovsky Prospect, Build. 1, Moscow 117997
Russian Federation
44, Vavilov St., Build. 2, Moscow 119333
27, Lomonosovsky Prospect, Build. 1, Moscow 117997
O. A. Gromova
Institute of Pharmacoinformatics, Federal Research Center «Informatics and Management», Russian Academy of Sciences; Center for Big Data Storage and Analysis, National Center for Digital Economy, M.V. Lomonosov Moscow State University
Russian Federation
Russian Federation
Olga Alekseevna Gromova
44, Vavilov St., Build. 2, Moscow 119333
27, Lomonosovsky Prospect, Build. 1, Moscow 117997
L. V. Stakhovskaya
N.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia
Russian Federation
1, Ostrovityanov St., Moscow 117997
Russian Federation
1, Ostrovityanov St., Moscow 117997
V. A. Semenov
Kemerovo State Medical University, Ministry of Health of Russia
Russian Federation
22a, Voroshilov St., Kemerovo 650029
Russian Federation
22a, Voroshilov St., Kemerovo 650029
I. A. Shchukin
N.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia
Russian Federation
1, Ostrovityanov St., Moscow 117997
Russian Federation
1, Ostrovityanov St., Moscow 117997
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Review
Supporting agencies: The investigation has been conducted under Russian Foundation for Basic Research Grants No. 20-07-00537 and No. 19-07-00356.
For citations:
Torshin I.Yu., Gromova O.A., Stakhovskaya L.V., Semenov V.A., Shchukin I.A. Chemotranscriptome analysis indicates the neurotrophic and neuromodulator effects of a citicoline molecule. Neurology, Neuropsychiatry, Psychosomatics. 2020;12(4):91-99. (In Russ.) https://doi.org/10.14412/2074-2711-2020-4-91-99
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