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Association of carriers of single nucleotide polymorphisms rs1143634 and rs16944 of the Il-1B gene and rs6265 of the BDNF gene with temporal lobe epilepsy

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Abstract

Temporal lobe epilepsy (TLE) is one of the most common and refractory forms of epilepsy, with a different etiology. According to experimental and clinical studies, the transformation of the normal pattern of brain neuron activity into paroxysmal one 3 is accompanied by changes in the expression of cytokines and neurotrophins in the hippocampus and temporal cortex. Modulation of the expression of the neurotrophic brain factor (Brain-Derived Neurotrophic Factor, BDNF) may be associated with the carrier of the single nucleotide polymorphism (SNP) of the rs6265 BDNF gene. The research groups have shown increased expression of BDNF in the hippocampus and temporal cortex in patients with drug resistant epilepsy. Independent studies have demonstrated the role of the Il1B gene, encoding pro-inflammatory cytokine interleukin (IL) 1β, in the development of inflammatory reactions and structural mediobasal temporal epilepsy with hippocampal sclerosis.

The purpose of the research is to study the association of the carriers of the SNP rs16944 and rs1143634 of the IL1B gene and rs6265 of the BDNF gene with the development of temporal lobe epilepsy.

Patients and methods. We carried out a molecular genetic study of the carrier of SNPs rs1143634 and rs16944 of the Il1B and rs6265 gene of the BDNF gene using real-time polymerase chain reaction (RT-PCR) in 84 patients with TLE and 203 Caucasian healthy volunteers living in the Siberian Federal Region.

Results. The carriage of a high-producing allele C (odds ratio, OR 2.01; 95% confidence interval, CI 1.31–3.08; p = 0.001) and the homozygous genotype CC (OR 2.48; 95% CI 1.47–4, 17; p = 0.001) of SNPs of the IL1B gene (rs1143634) are statistically significantly associated with the development of TLE in the population under study. No statistically significant differences in the carriers of the SNPs of the Il1B (rs1143634 and rs16944) gene and BDNF (rs6265) with the clinical features and course of TLE were revealed (p> 0.05). The association of carriers of SNPs rs6265 of the BDNF gene with the development of TLE was not detected (χ2 = 0.3; p = 0.86) as well.

Conclusion The association of the high-producing allele C carrier and the homozygous genotype CC (rs1143634) of the IL1B gene with TLE has been established. 

About the Authors

Y. S. Panina
V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University
Russian Federation
660022, Krasnoyarsk


D. V. Dmitrenko
V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University
Russian Federation
660022, Krasnoyarsk


N. A. Shnayder
V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University; V.M. Bekhterev National Medical Research Center of Psychiatry and Neurology
Russian Federation

660022, Krasnoyarsk;

St Petersburg



E. V. Egorova
V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University
Russian Federation
660022, Krasnoyarsk


A. A. Usoltseva
V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University
Russian Federation
660022, Krasnoyarsk


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For citation:


Panina Y.S., Dmitrenko D.V., Shnayder N.A., Egorova E.V., Usoltseva A.A. Association of carriers of single nucleotide polymorphisms rs1143634 and rs16944 of the Il-1B gene and rs6265 of the BDNF gene with temporal lobe epilepsy. Neurology, Neuropsychiatry, Psychosomatics. 0;. (In Russ.)

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ISSN 2074-2711 (Print)
ISSN 2310-1342 (Online)