nnpNeurology, Neuropsychiatry, PsychosomaticsНеврология, нейропсихиатрия, психосоматика2074-27112310-1342"IMA-Press", LLC10.14412/2074-2711-2022-5-28-35nnp-1888Research ArticleORIGINAL INVESTIGATIONSОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯ И МЕТОДИКИStudy of the role of carriage of single nucleotide variants of the IL-1β, TNFA, BDNF, NTRK-2 genes in the development and clinical features of temporal lobe epilepsyИсследование роли носительства однонуклеотидных вариантов генов IL-1β, TNFА, BDNF, NTRK-2 в развитии и клинических особенностях височной эпилепсииhttps://orcid.org/0000-0001-5204-7482ПанинаЮ. С.PaninaYu. S.

660022, Красноярск, ул. Партизана Железняка, 1

1, Partizana Zheleznyaka St., Krasnoyarsk 660022

https://orcid.org/0000-0001-9946-2878ДоморацкаяЕ. А.DomoratskayaE. A.

660022, Красноярск, ул. Партизана Железняка, 1

1, Partizana Zheleznyaka St., Krasnoyarsk 660022

e.domorats@yandex.ruПарамоноваА. И.ParamonovaA. I.

660022, Красноярск, ул. Партизана Железняка, 1

1, Partizana Zheleznyaka St., Krasnoyarsk 660022

https://orcid.org/0000-0003-4639-6365ДмитренкоД. В.DmitrenkoD. V.

660022, Красноярск, ул. Партизана Железняка, 1

1, Partizana Zheleznyaka St., Krasnoyarsk 660022

ФГБОУ ВО «Красноярский государственный медицинский университет им. проф. В.Ф. Войно-Ясенецкого» Минздрава РоссииРоссияV.F. Voyno-Yasenetsky Krasnoyarsk Medical State University, Ministry of Health of RussiaRussian Federation2022211020221452835Copyright © Panina Y.S., Domoratskaya E.A., Paramonova A.I., Dmitrenko D.V., 20222022Панина Ю.С., Доморацкая Е.А., Парамонова А.И., Дмитренко Д.В.Panina Y.S., Domoratskaya E.A., Paramonova A.I., Dmitrenko D.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://nnp.ima-press.net/nnp/article/view/1888

Temporal lobe epilepsy (TE) is the most common form of focal epilepsy in adults with a high rate of drug-resistant course. In the Russian Federation studies of the contribution of the carriage of single nucleotide variants of genes (SNGs) encoding proteins of neuroinflammation and neurodegeneration to the development of TE have not been previously carried out.

Objective

Objective: to study the association of SNGs rs16944 and rs1143634 of the IL-1β gene, rs1800629 of the TNFA gene, rs6265 of the BDNF gene, rs3780645 of the NTRK-2 gene with the risk of development, clinical and neuroimaging features of TE.

Patients and methods

Patients and methods. The study included 166 patients with TE and 203 healthy volunteers living in the Siberian Federal District. The study included clinical, neurophysiological, neuroradiological, and laboratory work-up. Investigation of the carriage of SNGs rs16944 (-511T/C) and rs1143634 (+3954C/T) of the IL-1β gene, rs1800629 (G-308A) of the TNFA gene, rs6265 (G/A) of the BDNF gene, rs3780645 (C/T) and rs2289656 (C/T) of the NTRK-2 gene was carried out by real-time polymerase chain reaction. Results and discussion. The prognostically unfavorable role of carriage of the A allele and the GA rs1800629 genotype of the TNFA gene in the development of TE, the GA rs6265 genotype of the BDNF gene in the development of TE with hippocampal sclerosis was established. Carrying the genotype AA rs1800629 of the TNFA gene in patients with TE reduces the risk of polytherapy with antiepileptic drugs.

Conclusion

Conclusion. The study of neuroinflammation and neurodegeneration processes is important both from a physiological point of view and from the point of view of searching for the TE development markers, which make it possible to predict and evaluate the rate of disease progression, help to determine the tactics of treatment, and evaluate its effectiveness. In this regard, at present, the identification of potential genetic markers remains a task of high priority. 

Височная эпилепсия (ВЭ) является самой распространенной формой фокальной эпилепсии у взрослых с высокой частотой фармакорезистентного течения. Исследования вклада носительства однонуклеотидных вариантов (ОНВ) генов, кодирующих белки нейровоспаления и нейродегенерации, в развитие ВЭ в Российской Федерации ранее не проводились.

Цель исследования – изучение ассоциации ОНВ rs16944 и rs1143634 гена IL-1β, rs1800629 гена TNFА, rs6265 гена BDNF, rs3780645 гена NTRK-2 с риском развития, клиническими и нейровизуализационными особенностями ВЭ.

Пациенты и методы

Пациенты и методы. В исследование включено 166 пациентов с ВЭ и 203 здоровых добровольца, проживающих в Сибирском федеральном округе. Исследование включало клинические, нейрофизиологические, нейрорадиологические, лабораторные методы исследования. Исследование носительства ОНВ rs16944 (-511T/C) и rs1143634 (+3954C/T) гена IL-1β, rs1800629 (G-308A) гена TNFA, rs6265 (G/A) гена BDNF, rs3780645 (C/T) и rs2289656 (C/T) гена NTRK-2 проведено методом полимеразной цепной реакции в режиме реального времени.

Результаты и обсуждение

Результаты и обсуждение. Установлена прогностически неблагоприятная роль носительства аллеля А и генотипа GA rs1800629 гена TNFA в развитии ВЭ, генотипа GA rs6265 гена BDNF с развитием ВЭ с гиппокампальным склерозом. Носительство генотипа АА rs1800629 гена TNFA у пациентов с ВЭ снижает риск политерапии противоэпилептическими препаратами.

Заключение

Заключение. Изучение процессов нейровоспаления и нейродегенерации важно как с физиологической точки зрения, так и с точки зрения поиска маркеров развития ВЭ, позволяющих предсказать и оценить темп прогрессирования заболевания, помочь в определении тактики лечения и оценке его эффективности. В связи с этим в настоящее время выявление потенциальных генетических маркеров остается крайне актуальной задачей. 

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The authors declare that there are no conflicts of interest present.